stemmacstm ipsc mrna reprogramming kit (Miltenyi Biotec)
Structured Review

Stemmacstm Ipsc Mrna Reprogramming Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stemmacs+mrna+reprogramming+kit/StemMACS+iPSC+mRNA+Reprogramming+Kit%2C+human/pmc13322296-78-28-33
Average 94 stars, based on 2 article reviews
Images
1) Product Images from "Human iPSCs derived from cryopreserved testicular somatic cells enable germline regeneration in childhood cancer survivors"
Article Title: Human iPSCs derived from cryopreserved testicular somatic cells enable germline regeneration in childhood cancer survivors
Journal: Human Reproduction Open
doi: 10.1093/hropen/hoag054
Figure Legend Snippet: Overview of the experimental layout: focus on primary testicular somatic cell cultures and reprogramming . ( A ) Schematic overview of the experimental procedures. ( B ) Non-genome-integrating, feeder-free reprogramming of human induced pluripotent stem cells (hiPSCs) from primary testicular somatic cells, with the corresponding time required for each step. Left panel: Phase-contrast images of confluent primary testicular somatic cell cultures approximately 1 week after isolation from the cryopreserved tissue; Middle panel: Emerging hiPSC colonies following the mRNA-based reprogramming protocol; Right panel: Expansion of the isolated hiPSC clones NFKI001 at passage 8 (p8) and NFKI002 at p15 from Patients 1 and 2, respectively. Scale bars, 500 μm. Figure (A) was created using BioRender (Alves Lopes, J. P. (2026); https://BioRender.com/gv6k171 ).
Techniques Used: Isolation, Clone Assay
Related Articles
Generated:Article Title: Small fibre neuropathy in Fabry disease: a human-derived neuronal in vitro disease model Article Snippet: Briefly, dermis and epidermis were mechanically separated and the dermal part was collected in fibroblast cultivation medium (DMEM/F12 + 100 U/ml penicillin 100 μg/ml streptomycin [pen/strep; both: Thermo Fisher Scientific, Waltham, MA, USA] + 10% fetal calf serum [FCS; Merck, Darmstadt, Germany]). .. All cell lines were cultivated at 37°C with 5% CO 2 (v/v). iPSC were generated using the StemRNA 3 rd Gen reprogramming kit (Reprocell, Maryland, USA) for all male cell lines (FD-1, FD-2, Ctrl) and the Article Title: Small fibre neuropathy in Fabry disease: a human-derived neuronal in vitro disease model and pilot data Article Snippet: Briefly, dermis and epidermis were mechanically separated, and the dermal part was collected in fibroblast cultivation medium [Dulbecco's Modified Eagle Medium, DMEM/F12 + 100 U/ml penicillin 100 μg/ml streptomycin (pen/strep; both: Thermo Fisher Scientific, Waltham, MA, USA) + 10% fetal calf serum (FCS; Merck, Darmstadt, Germany)]. .. All cell lines were cultivated at 37°C with 5% CO 2 (v/v). iPSC were generated using the StemRNA 3rd Gen reprogramming kit (Reprocell, Beltsville, MD, USA) for all male cell lines (FD-1, FD-2 and Ctrl) and the Transfection:Article Title: Human iPSC-based neurodevelopmental models of globoid cell leukodystrophy uncover patient- and cell type-specific disease phenotypes Article Snippet: .. The Article Title: Human iPSC-based neurodevelopmental models of globoid cell leukodystrophy uncover patient- and cell type-specific disease phenotypes Article Snippet: Reprogramming factors were delivered as modified mRNAs (mmRNAs) taking advantage of a microfluidic system ( ). .. The |