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stemmacstm ipsc mrna reprogramming kit  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec stemmacstm ipsc mrna reprogramming kit
    Overview of the experimental layout: focus on primary testicular somatic cell cultures and <t>reprogramming</t> . ( A ) Schematic overview of the experimental procedures. ( B ) Non-genome-integrating, feeder-free reprogramming of human induced pluripotent stem cells (hiPSCs) from primary testicular somatic cells, with the corresponding time required for each step. Left panel: Phase-contrast images of confluent primary testicular somatic cell cultures approximately 1 week after isolation from the cryopreserved tissue; Middle panel: Emerging hiPSC colonies following the <t>mRNA-based</t> reprogramming protocol; Right panel: Expansion of the isolated hiPSC clones NFKI001 at passage 8 (p8) and NFKI002 at p15 from Patients 1 and 2, respectively. Scale bars, 500 μm. Figure (A) was created using BioRender (Alves Lopes, J. P. (2026); https://BioRender.com/gv6k171 ).
    Stemmacstm Ipsc Mrna Reprogramming Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stemmacs+mrna+reprogramming+kit/StemMACS+iPSC+mRNA+Reprogramming+Kit%2C+human/pmc13322296-78-28-33
    Average 94 stars, based on 2 article reviews
    stemmacstm ipsc mrna reprogramming kit - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Human iPSCs derived from cryopreserved testicular somatic cells enable germline regeneration in childhood cancer survivors"

    Article Title: Human iPSCs derived from cryopreserved testicular somatic cells enable germline regeneration in childhood cancer survivors

    Journal: Human Reproduction Open

    doi: 10.1093/hropen/hoag054

    Overview of the experimental layout: focus on primary testicular somatic cell cultures and reprogramming . ( A ) Schematic overview of the experimental procedures. ( B ) Non-genome-integrating, feeder-free reprogramming of human induced pluripotent stem cells (hiPSCs) from primary testicular somatic cells, with the corresponding time required for each step. Left panel: Phase-contrast images of confluent primary testicular somatic cell cultures approximately 1 week after isolation from the cryopreserved tissue; Middle panel: Emerging hiPSC colonies following the mRNA-based reprogramming protocol; Right panel: Expansion of the isolated hiPSC clones NFKI001 at passage 8 (p8) and NFKI002 at p15 from Patients 1 and 2, respectively. Scale bars, 500 μm. Figure (A) was created using BioRender (Alves Lopes, J. P. (2026); https://BioRender.com/gv6k171 ).
    Figure Legend Snippet: Overview of the experimental layout: focus on primary testicular somatic cell cultures and reprogramming . ( A ) Schematic overview of the experimental procedures. ( B ) Non-genome-integrating, feeder-free reprogramming of human induced pluripotent stem cells (hiPSCs) from primary testicular somatic cells, with the corresponding time required for each step. Left panel: Phase-contrast images of confluent primary testicular somatic cell cultures approximately 1 week after isolation from the cryopreserved tissue; Middle panel: Emerging hiPSC colonies following the mRNA-based reprogramming protocol; Right panel: Expansion of the isolated hiPSC clones NFKI001 at passage 8 (p8) and NFKI002 at p15 from Patients 1 and 2, respectively. Scale bars, 500 μm. Figure (A) was created using BioRender (Alves Lopes, J. P. (2026); https://BioRender.com/gv6k171 ).

    Techniques Used: Isolation, Clone Assay

    Related Articles

    Generated:

    Article Title: Small fibre neuropathy in Fabry disease: a human-derived neuronal in vitro disease model
    Article Snippet: Briefly, dermis and epidermis were mechanically separated and the dermal part was collected in fibroblast cultivation medium (DMEM/F12 + 100 U/ml penicillin 100 μg/ml streptomycin [pen/strep; both: Thermo Fisher Scientific, Waltham, MA, USA] + 10% fetal calf serum [FCS; Merck, Darmstadt, Germany]). .. All cell lines were cultivated at 37°C with 5% CO 2 (v/v). iPSC were generated using the StemRNA 3 rd Gen reprogramming kit (Reprocell, Maryland, USA) for all male cell lines (FD-1, FD-2, Ctrl) and the StemMACS mRNA reprogramming kit (Miltenyi Biotec, Bergisch Gladbach, Germany) for the female Fabry cell line (FD-3; ). ..

    Article Title: Small fibre neuropathy in Fabry disease: a human-derived neuronal in vitro disease model and pilot data
    Article Snippet: Briefly, dermis and epidermis were mechanically separated, and the dermal part was collected in fibroblast cultivation medium [Dulbecco's Modified Eagle Medium, DMEM/F12 + 100 U/ml penicillin 100 μg/ml streptomycin (pen/strep; both: Thermo Fisher Scientific, Waltham, MA, USA) + 10% fetal calf serum (FCS; Merck, Darmstadt, Germany)]. .. All cell lines were cultivated at 37°C with 5% CO 2 (v/v). iPSC were generated using the StemRNA 3rd Gen reprogramming kit (Reprocell, Beltsville, MD, USA) for all male cell lines (FD-1, FD-2 and Ctrl) and the StemMACS mRNA reprogramming kit (Miltenyi Biotec, Bergisch Gladbach, Germany) for the female Fabry cell line (FD-3 ). ..

    Transfection:

    Article Title: Human iPSC-based neurodevelopmental models of globoid cell leukodystrophy uncover patient- and cell type-specific disease phenotypes
    Article Snippet: .. The StemMACS mRNA Reprogramming Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) was used for mRNA transfection according to the manufacturer’s instructions. ..

    Article Title: Human iPSC-based neurodevelopmental models of globoid cell leukodystrophy uncover patient- and cell type-specific disease phenotypes
    Article Snippet: Reprogramming factors were delivered as modified mRNAs (mmRNAs) taking advantage of a microfluidic system ( ). .. The StemMACS mRNA Reprogramming Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) was used for mRNA transfection according to the manufacturer instructions. ..



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    Miltenyi Biotec stemmacstm ipsc mrna reprogramming kit
    Overview of the experimental layout: focus on primary testicular somatic cell cultures and <t>reprogramming</t> . ( A ) Schematic overview of the experimental procedures. ( B ) Non-genome-integrating, feeder-free reprogramming of human induced pluripotent stem cells (hiPSCs) from primary testicular somatic cells, with the corresponding time required for each step. Left panel: Phase-contrast images of confluent primary testicular somatic cell cultures approximately 1 week after isolation from the cryopreserved tissue; Middle panel: Emerging hiPSC colonies following the <t>mRNA-based</t> reprogramming protocol; Right panel: Expansion of the isolated hiPSC clones NFKI001 at passage 8 (p8) and NFKI002 at p15 from Patients 1 and 2, respectively. Scale bars, 500 μm. Figure (A) was created using BioRender (Alves Lopes, J. P. (2026); https://BioRender.com/gv6k171 ).
    Stemmacstm Ipsc Mrna Reprogramming Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stemmacs+mrna+reprogramming+kit/StemMACS+iPSC+mRNA+Reprogramming+Kit%2C+human/pmc13322296-78-28-33
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    Miltenyi Biotec stemmacs mrna reprogramming kit
    Overview of the experimental layout: focus on primary testicular somatic cell cultures and <t>reprogramming</t> . ( A ) Schematic overview of the experimental procedures. ( B ) Non-genome-integrating, feeder-free reprogramming of human induced pluripotent stem cells (hiPSCs) from primary testicular somatic cells, with the corresponding time required for each step. Left panel: Phase-contrast images of confluent primary testicular somatic cell cultures approximately 1 week after isolation from the cryopreserved tissue; Middle panel: Emerging hiPSC colonies following the <t>mRNA-based</t> reprogramming protocol; Right panel: Expansion of the isolated hiPSC clones NFKI001 at passage 8 (p8) and NFKI002 at p15 from Patients 1 and 2, respectively. Scale bars, 500 μm. Figure (A) was created using BioRender (Alves Lopes, J. P. (2026); https://BioRender.com/gv6k171 ).
    Stemmacs Mrna Reprogramming Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
    stemmacs mrna reprogramming kit - by Bioz Stars, 2026-09
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    Miltenyi Biotec mrna reprogramming kit
    Overview of the experimental layout: focus on primary testicular somatic cell cultures and <t>reprogramming</t> . ( A ) Schematic overview of the experimental procedures. ( B ) Non-genome-integrating, feeder-free reprogramming of human induced pluripotent stem cells (hiPSCs) from primary testicular somatic cells, with the corresponding time required for each step. Left panel: Phase-contrast images of confluent primary testicular somatic cell cultures approximately 1 week after isolation from the cryopreserved tissue; Middle panel: Emerging hiPSC colonies following the <t>mRNA-based</t> reprogramming protocol; Right panel: Expansion of the isolated hiPSC clones NFKI001 at passage 8 (p8) and NFKI002 at p15 from Patients 1 and 2, respectively. Scale bars, 500 μm. Figure (A) was created using BioRender (Alves Lopes, J. P. (2026); https://BioRender.com/gv6k171 ).
    Mrna Reprogramming Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stemmacs+mrna+reprogramming+kit/StemMACS+iPSC+mRNA+Reprogramming+Kit%2C+human/pmc06486358-542-19-22
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    Image Search Results


    Overview of the experimental layout: focus on primary testicular somatic cell cultures and reprogramming . ( A ) Schematic overview of the experimental procedures. ( B ) Non-genome-integrating, feeder-free reprogramming of human induced pluripotent stem cells (hiPSCs) from primary testicular somatic cells, with the corresponding time required for each step. Left panel: Phase-contrast images of confluent primary testicular somatic cell cultures approximately 1 week after isolation from the cryopreserved tissue; Middle panel: Emerging hiPSC colonies following the mRNA-based reprogramming protocol; Right panel: Expansion of the isolated hiPSC clones NFKI001 at passage 8 (p8) and NFKI002 at p15 from Patients 1 and 2, respectively. Scale bars, 500 μm. Figure (A) was created using BioRender (Alves Lopes, J. P. (2026); https://BioRender.com/gv6k171 ).

    Journal: Human Reproduction Open

    Article Title: Human iPSCs derived from cryopreserved testicular somatic cells enable germline regeneration in childhood cancer survivors

    doi: 10.1093/hropen/hoag054

    Figure Lengend Snippet: Overview of the experimental layout: focus on primary testicular somatic cell cultures and reprogramming . ( A ) Schematic overview of the experimental procedures. ( B ) Non-genome-integrating, feeder-free reprogramming of human induced pluripotent stem cells (hiPSCs) from primary testicular somatic cells, with the corresponding time required for each step. Left panel: Phase-contrast images of confluent primary testicular somatic cell cultures approximately 1 week after isolation from the cryopreserved tissue; Middle panel: Emerging hiPSC colonies following the mRNA-based reprogramming protocol; Right panel: Expansion of the isolated hiPSC clones NFKI001 at passage 8 (p8) and NFKI002 at p15 from Patients 1 and 2, respectively. Scale bars, 500 μm. Figure (A) was created using BioRender (Alves Lopes, J. P. (2026); https://BioRender.com/gv6k171 ).

    Article Snippet: Upon reaching confluency, cells were seeded at 2000 cells/cm 2 in 35 mm dishes on day-3 and transfected twice daily from day 0 to day 4 using the StemMACSTM iPSC mRNA Reprogramming Kit (Miltenyi Biotec, Bergisch Gladbach, Germany, #130-132-990).

    Techniques: Isolation, Clone Assay